Analyze > Analyze particles > Show > Bare Outlines. This table can be obtained within ImageJ using the Plugins Shortcuts List Shortcuts command. Run("iterative PIV(Cross-correlation).", "piv1=64 piv2=32 piv3=0 what= noise=0. Threshold the image (Image > Adjust > Threshold) to outline all the regions and click Apply. Also note that, except when using the Text Tool, you do not need to hold down the control key to use a keyboard shortcut, unless Require control key for shortcuts in Edit Options Misc is checked. Run("Images to Stack", "method= name= title=-1 use keep") Run("Bandpass Filter.", "filter_large=120 filter_small=3 suppress=None tolerance=5 autoscale saturate") / make dialog window with instructions /// Run("Bio-Formats Importer", "open=strainpath autoscale color_mode=Default view=Hyperstack stack_order=XYCZT") Run("Bio-Formats Importer", "open=relaxpath autoscale color_mode=Default view=Hyperstack stack_order=XYCZT") Run("Bio-Formats Importer", "open=DICpath autoscale color_mode=Default view=Hyperstack stack_order=XYCZT") Please see my code below - the part I’m stuck on is demarcated ///like this/// I can do most of this, but I can’t see how to open a dialog and have a while loop check that the dialog is still open. I want to be able to open a dialog that asks the user to define the locations of one or more cells in the image (with the mouse), creates square ROIs of defined size centered on each point and saves them to the ROI manager, and then continues the macro when the “ok” button is pushed. This plugin can perform Sholl directly on 2D and 3D grayscale images of isolated neurons. I’m trying to write a macro to streamline my traction force analysis. The Sholl technique is used to describe neuronal arbors.
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